Development of culture media for the construction of vascularized adipose tissue and vascularized 3D full-skin equivalents in vitro
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A first vascularized skin equivalent as an alternative to animal experimentation.
Tissue-engineered skin equivalents mimic key aspects of the human skin, and can thus be employed as wound coverage for large skin defects or as in vitro test systems as an alternative to animal models. However, current skin equivalents lack a functional vasculature limiting clinical and research applications. This study demonstrates the generation of a vascularized skin equivalent with a perfus...
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Management of long bone defects occurring in accidents or war injuries is a difficult problem, From 1978 to 1986, 11 patients were treated for long bone defects of the humerus ( 10 cases) and radius (one case), using free vascularized fibular transfer by microsurgical technique .The patients were followed for 4 months and in all of them, selective angiography and scanning revealed exellent...
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Survival of functional tissue constructs of clinically relevant size depends on the formation of an organized and uniformly distributed network of blood vessels and capillaries. The lack of such vasculature leads to spatio-temporal gradients in oxygen, nutrients and accumulation of waste products inside engineered tissue constructs resulting in negative biological events at the core of the scaf...
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Objective(s) Some investigation has indicated that adipose-derived stem cells possess different surface epitopes and differentiation potential according to the localization of fat pad from which the cells were derived. In the present study proliferation capacity and aging of such cells were explored. Materials and Methods Adherent cells were isolated from the collagenase digests of adipose tiss...
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